abstract：:A highly efficient method of transposon mutagenesis was developed for genetic analysis of Xanthobacter autotrophicus Py2. The method makes use of a transposon delivery vector that encodes a hyperactive Tn 5 transposase that is 1,000-fold more active than the wild-type transposase. In this construct, the transposase is...

journal_title：Archives of microbiology

authors： Larsen RA,Wilson MM,Guss AM,Metcalf WW

更新日期：2002-09-01 00:00:00

abstract：:In order to investigate the role of the putative epimerase function of the beta-oxidation multienzyme complex (FadBA) in the provision of (R)-3-hydroxyacyl-CoA thioesters for medium-chain-length polyhydroxyalkanoate (PHA(MCL)) biosynthesis, the fadBA(Po) operon of Pseudomonas oleovorans was cloned and characterized. T...

journal_title：Archives of microbiology

authors： Fiedler S,Steinbüchel A,Rehm BH

更新日期：2002-08-01 00:00:00

abstract：:The methanotrophic bacterium Methylomicrobium album BG8 uses methane as a sole source of carbon and energy. This bacterium forms an extensive intracytoplasmic membrane. The first enzymes of the methane oxidation pathway are the membrane-bound particulate methane monooxygenase and the periplasmic methanol dehydrogenase...

journal_title：Archives of microbiology

authors： Brantner CA,Remsen CC,Owen HA,Buchholz LA,Perille Collins ML

更新日期：2002-07-01 00:00:00

abstract：:The proteins in the culture supernatant (exoproteins) from Streptococcus pyogenes serotype M1 were separated by two-dimensional gel electrophoresis, and their N-terminal amino acid sequences were determined. The amino acid sequences were compared to sequences in the S. pyogenes genome database. The coding sequence sho...

journal_title：Archives of microbiology

authors： Hasegawa T,Torii K,Hashikawa S,Iinuma Y,Ohta M

更新日期：2002-06-01 00:00:00

abstract：:Escherichia coli, Salmonella enterica, Klebsiella pneumoniaeand Klebsiella oxytocawere found to contain two D-tagatose 1,6-bisphosphate (TagBP)-specific aldolases involved in catabolism of galactitol (genes gatY gatZ) and of N-acetyl-galactosamine and D-galactosamine (genes kbaY kbaZ,also called agaY agaZ). The two al...

journal_title：Archives of microbiology

authors： Brinkkötter A,Shakeri-Garakani A,Lengeler JW

更新日期：2002-05-01 00:00:00

abstract：:Saccharomyces cerevisiaepresents two alternative vegetative forms of growth, switching between yeast forms to pseudohyphal forms depending on the specific environmental conditions. To identify genes involved in cell wall morphogenesis, a haploid S. cerevisiae monomorphic mutant, W27, which exhibits pseudohyphal growth...

journal_title：Archives of microbiology

authors： Cañizares JV,Pallotti C,Saínz-Pardo I,Iranzo M,Mormeneo S

更新日期：2002-04-01 00:00:00

abstract：:A novel thermotolerant strain of the achlorophyllous micro-alga Prototheca was isolated from a hot spring. The isolate was found to produce an appreciable amount of ethanol and CO2 from glucose under anoxic conditions at both 25 and 40 degrees C; this type of alcohol fermentation has not yet been reported in the genus...

journal_title：Archives of microbiology

authors： Ueno R,Urano N,Suzuki M,Kimura S

更新日期：2002-03-01 00:00:00

abstract：:Helcococcus kunzii is a gram-positive, catalase-negative opportunist. The organism has been isolated from the lower extremities and breast masses of several patients. A clinical isolate of Helcococcus kunzii was shown to possess a hemagglutinin-lectin with a specificity for N-acetylglucosamine and lactose, two structu...

journal_title：Archives of microbiology

authors： Stavri H,Beveridge TJ,Moyles D,Athamna A,Doyle RJ

更新日期：2002-02-01 00:00:00

abstract：:Bacillus subtilis strain 168 encodes two flavocytochromes P450, Cyp102A2 and Cyp102A3. The cyp102A3 gene is preceded by, and organized in an operon with, a gene for a transcriptional regulator, encoded by fatR. The paralogous gene, cyp102A2, is most likely transcribed as a mono-cistronic message. We show that fatR enc...

journal_title：Archives of microbiology

authors： Gustafsson MC,Palmer CN,Wolf CR,von Wachenfeldt C

更新日期：2001-12-01 00:00:00

abstract：:Triacylglycerol granules synthesized and accumulated by Rhodococcus opacus and Rhodococcus ruber were isolated by glycerol density gradient centrifugation. Whereas only one type of granule could be isolated from R. opacus, two types of granules with different specific densities were isolated from R. ruber. Both types ...

journal_title：Archives of microbiology

authors： Kalscheuer R,Wältermann M,Alvarez M,Steinbüchel A

更新日期：2001-12-01 00:00:00

abstract：:We previously cloned the sigH gene encoding a stress-response sigma factor sigma(H) in Streptomyces coelicolor A3(2), located in an operon with the gene encoding proposed anti-sigma factor UshX. To clarify the in vivo function of sigma(H), a stable null mutant of sigH was prepared by homologous recombination. This mut...

journal_title：Archives of microbiology

authors： Sevciková B,Benada O,Kofronova O,Kormanec J

更新日期：2001-12-01 00:00:00

abstract：:Type I signal peptidases are responsible for the proteolytic cleavage of the signal peptide of secreted proteins. In the gram-positive bacterium Streptomyces lividans, four adjacent genes (sipW, sipX, sipY and sipZ) were isolated encoding putative type I signal peptidases. In this work, the different sip genes were cl...

journal_title：Archives of microbiology

authors： Geukens N,Parro V,Rivas LA,Mellado RP,Anné J

更新日期：2001-11-01 00:00:00

abstract：:The starvation survivability of seven Thermococcus strains isolated from four Japanese oil reservoirs was compared with that of Thermococcus strains from marine hydrothermal fields. 16S rDNA analyses showed the isolates to be closely related to Thermococcus litoralis. Growth of the isolates was dependent on amino acid...

journal_title：Archives of microbiology

authors： Takahata Y,Hoaki T,Maruyama T

更新日期：2001-10-01 00:00:00

abstract：:Carotenogenic mutants of Corynebacterium glutamicum were analyzed for their carotenoid content. Mutant MV10 accumulated the same carotenoids as the wild-type, decaprenoxanthin, decaprenoxanthin monoglucoside, and (2R,6R,2'R,6'R)-decaprenoxanthin di-(beta-D)-glucoside, but in three-fold higher amounts. In addition, dec...

journal_title：Archives of microbiology

authors： Krubasik P,Takaichi S,Maoka T,Kobayashi M,Masamoto K,Sandmann G

更新日期：2001-09-01 00:00:00

abstract：:The glutamate catabolism of three thermophilic syntrophic anaerobes was compared based on the combined use of [(13)C] glutamate NMR measurements and enzyme activity determinations. In some cases the uptake of intermediates from different pathways was studied. The three organisms, Caloramator coolhaasii, Thermanaerovib...

journal_title：Archives of microbiology

authors： Plugge CM,van Leeuwen JM,Hummelen T,Balk M,Stams AJ

更新日期：2001-07-01 00:00:00

abstract：:The induction of the enzyme activities involved in butane metabolism in Pseudomonas butanovora was characterized. P. butanovora was grown on butane or its metabolites, both singly and in mixtures with other growth substrates. Cells grown in each of the butane metabolites readily consumed the growth substrate and downs...

journal_title：Archives of microbiology

authors： Sayavedra-Soto LA,Byrd CM,Arp DJ

更新日期：2001-07-01 00:00:00

abstract：:RpoS, the alternative sigma factor sigma(s), is important for bacterial survival under extreme conditions. Many enterobacteria are opportunistic human pathogens and their ability to survive in a changing environment could be an essential step for their virulence. To determine the presence of this gene in enteric bacte...

journal_title：Archives of microbiology

authors： Martínez-García E,Tormo A,Navarro-Llorens JM

更新日期：2001-06-01 00:00:00

abstract：:Halometabolites are compounds that are commonly found in nature and they are produced by many different organisms. Whereas bromometabolites can mainly be found in the marine environment, chlorometabolites are predominately produced by terrestrial organisms; iodo- and fluorocompounds are only produced infrequently. The...

journal_title：Archives of microbiology

authors： van Pée KH

更新日期：2001-04-01 00:00:00

abstract：:Regulation of transcription of the Escherichia coli moe operon, which codes for proteins connecting molybdate metabolism, molybdopterin synthesis, and apomolybdoenzyme synthesis, was investigated. Expression of the moe operon was independent of genes coding for molybdate transport and Mo-cofactor biosynthesis. Express...

journal_title：Archives of microbiology

authors： Hasona A,Self WT,Shanmugam KT

更新日期：2001-03-01 00:00:00

abstract：:In beta-lactam-antibiotic-producing fungi, such as Aspergillus (Emericella) nidulans, L-alpha-aminoadipic acid is the branching point of the lysine and penicillin biosynthesis pathways. To obtain a deeper insight into the regulation of lysine biosynthesis genes, the regulation of the A. nidulans lysF gene, which encod...

journal_title：Archives of microbiology

authors： Weidner G,Steidl S,Brakhage AA

更新日期：2001-02-01 00:00:00

abstract：:To confirm the existence in nature of Salmonella enteritidis strains of different degrees of virulence and to elucidate the mechanisms underlying the effects of such strains on the epithelial barrier function, the consequences of infection of Caco-2 cells and HEp-2 cells with 15 S. enteritidis strains in a chicken inf...

journal_title：Archives of microbiology

authors： Solano C,Sesma B,Alvarez M,Urdaneta E,Garcia-Ros D,Calvo A,Gamazo C

更新日期：2001-01-01 00:00:00

abstract：:A new psychrophilic, anaerobic, acetogenic bacterium from the tundra wetland soil of Polar Ural is described. The organism fermented H2/CO2, formate, methanol, and several sugars to acetate as the sole end-product. The temperature range for growth was 1-30 degrees C with an optimum at 20 degrees C. The bacterium showe...

journal_title：Archives of microbiology

authors： Simankova MV,Kotsyurbenko OR,Stackebrandt E,Kostrikina NA,Lysenko AM,Osipov GA,Nozhevnikova AN

更新日期：2000-12-01 00:00:00

abstract：:The symbiotic bacteria Bradyrhizobium japonicum and Sinorhizobium meliloti, and the purple photosynthetic bacteria Rhodobacter capsulatus, Rhodovulum sulfidophilum, Roseobacter denitrificans and Rhodobacter sphaeroides possess homologous two-component regulatory systems, namely RegSR, ActSR, RegBA and PrrBA. The respe...

journal_title：Archives of microbiology

authors： Emmerich R,Hennecke H,Fischer HM

更新日期：2000-11-01 00:00:00

abstract：:Recently it was found that the specific activity of H2-forming methylenetetrahydromethanopterin dehydrogenase (Hmd) in Methanothermobacter marburgensis (formerly Methanobacterium thermoautotrophicum strain Marburg) increased six-fold when the hydrogenotrophic archaeon was grown in chemostat culture under nickel-limite...

journal_title：Archives of microbiology

authors： Afting C,Kremmer E,Brucker C,Hochheimer A,Thauer RK

更新日期：2000-10-01 00:00:00

abstract：:Clostridium aminobutyricum ferments 4-aminobutyrate via succinic semialdehyde, 4-hydroxybutyrate, 4-hydroxybutyryl-CoA and crotonyl-CoA to acetate and butyrate. The genes coding for the enzymes that catalyse the interconversion of these intermediates are arranged in the order abfD (4-hydroxybutyryl-CoA dehydratase), a...

journal_title：Archives of microbiology

authors： Gerhardt A,Cinkaya I,Linder D,Huisman G,Buckel W

更新日期：2000-09-01 00:00:00

abstract：:The ability of Methylosinus trichosporium OB3b, expressing soluble methane monooxygenase, to oxidize a range of ortho-substituted biphenyls was examined to better understand how substituents affect both the rate and products of oxidation in comparison to biphenyl. Inhibition of oxidation was observed over the tested s...

journal_title：Archives of microbiology

authors： Lindner AS,Adriaens P,Semrau JD

更新日期：2000-07-01 00:00:00

abstract：:Sulfolane (tetrahydrothiophene-1,1-dioxide) is used in the Sulfinol process for natural gas sweetening. At many sour-gas processing plants spills, landfills and leakage from unlined surface storage ponds have contaminated groundwaters with sulfolane. Due to its high water solubility and mobility in aquifers, sulfolane...

journal_title：Archives of microbiology

authors： Greene EA,Beatty PH,Fedorak PM

更新日期：2000-07-01 00:00:00

abstract：:Methanotrophs are ubiquitous in the environment and play an important role in mitigating global warming due to methane. They are also potentially interesting for industrial applications such as production of bulk chemicals or bioremediation. The first step in the oxidation of methane is the conversion to methanol by m...

journal_title：Archives of microbiology

authors： Murrell JC,Gilbert B,McDonald IR

更新日期：2000-05-01 00:00:00

abstract：:Fermentative degradation of phenol was studied using a non-methanogenic, pasteurised enrichment culture containing two morphologically different bacteria. Phenol was fermented to benzoate, acetate and butyrate and their relative occurrence depended on the concentration of hydrogen. Proportionately more benzoate was fo...

journal_title：Archives of microbiology

authors： Karlsson A,Ejlertsson J,Svensson BH

更新日期：2000-05-01 00:00:00

abstract：:During a screening program intended to identify genes encoding enzymes typical for secondary metabolism in Sorangium cellulosum So ce90, an aromatic amino acid decarboxylase gene (ddc) was detected. Expression of ddc in Escherichia coli and subsequent enzyme assays with cell-free extracts confirmed the proposed functi...

journal_title：Archives of microbiology

authors： Müller R,Gerth K,Brandt P,Blöcker H,Beyer S

更新日期：2000-04-01 00:00:00

abstract：:Development of a transformation system in the n-alkane-assimilating diploid yeast Candida tropicalis requires an antibiotic resistance gene in order to establish a selectable marker. The resistance gene for hygromycin B has often been used as a selectable marker in yeast transformation. However, C. tropicalis harborin...

journal_title：Archives of microbiology

authors： Hara A,Ueda M,Misawa S,Matsui T,Furuhashi K,Tanaka A

更新日期：2000-03-01 00:00:00

abstract：:The diversity among 45 cyanobacterial isolates from 11 different Gunnera species originating from different geographical areas was examined. By means of polymerase chain reaction (PCR) fingerprinting with short tandemly repeated repetitive (STRR) sequences as primers, ten groups of symbiotic cyanobacteria and five uni...

journal_title：Archives of microbiology

authors： Nilsson M,Bergman B,Rasmussen U

更新日期：2000-02-01 00:00:00

abstract：:A monoclonal antibody obtained by immunization of mice with heat-killed cells of Listeria monocytogenes serotype 4d showed reactivity towards a protein (P45) from L. monocytogenes with an apparent molecular mass of 45 kDa. This protein was detected in the culture supernatant and at the cell surface of L. monocytogenes...

journal_title：Archives of microbiology

authors： Schubert K,Bichlmaier AM,Mager E,Wolff K,Ruhland G,Fiedler F

更新日期：2000-01-01 00:00:00

abstract：:The subtype of phosphofructokinase activity, either ATP-, ADP- or pyrophosphate-dependent, present in members of three genera from the Spirochaetales was investigated. The individual species/strains examined included Spirochaeta alkalica, S. asiatica, S. halophila, S. isovalerica, S. litoralis, S. zuelzerae, S. thermo...

journal_title：Archives of microbiology

authors： Ronimus RS,Morgan HW,Ding YR

更新日期：1999-12-01 00:00:00

abstract：:The anaerobic bacterium Desulfobacterium cetonicum oxidized m-cresol completely with sulfate as electron acceptor. During growth, 3-hydroxybenzylsuccinate (identified by gas chromatography/mass spectroscopy and by comparison of high-performance liquid chromatography retention time and UV spectrum with a chemically syn...

journal_title：Archives of microbiology

authors： Müller JA,Galushko AS,Kappler A,Schink B

更新日期：1999-11-01 00:00:00

abstract：:The carboxysomal polypeptides of Thiobacillus neapolitanus with apparent molecular masses of 85 and 130 kDa were isolated and subjected to N-terminal sequencing. The first 17 amino acids of the two peptides were identical. The sequence perfectly matched the deduced amino acid sequence of an open reading frame in the c...

journal_title：Archives of microbiology

authors： Baker SH,Lorbach SC,Rodriguez-Buey M,Williams DS,Aldrich HC,Shively JM

更新日期：1999-10-01 00:00:00

abstract：:An extremely thermophilic, sulfur-dependent archaeon, strain WT1, was isolated from a freshwater hot spring in the Lake Taupo area of North Island, New Zealand. The cells are flagellated, strictly anaerobic cocci that grow optimally at 85 degrees C and 5.4 g NaCl l(-1). The strain grows heterotrophically on complex pr...

journal_title：Archives of microbiology

authors： González JM,Sheckells D,Viebahn M,Krupatkina D,Borges KM,Robb FT

更新日期：1999-08-01 00:00:00

abstract：:Bacteriochlorophyll (BChl) c in whole cells of Chlorobium tepidum grown at 46 degrees C changed into bacteriopheophytin (BPhe) c within 10 days after reaching full growth. When a small amount of C. tepidum cells in which BChl c had been completely pheophytinized were transferred to a new culture medium, normal growth ...

journal_title：Archives of microbiology

authors： Tokita S,Hirota M,Frigaard NU,Shimada K,Matsuura K

更新日期：1999-07-01 00:00:00

abstract：:Glucose-limited continuous cultures were used to analyze sigmaB activity at decreasing growth rates. Expression of the sigmaB-dependent genes gsiB and ctc started to increase at a growth rate of 0.2 h-1, and both genes were induced approximately fivefold at a growth rate of 0.1 h-1 as compared to expression at the max...

journal_title：Archives of microbiology

authors： Schweder T,Kolyschkow A,Völker U,Hecker M

更新日期：1999-05-01 00:00:00

abstract：:Twelve genes involved in thiamin biosynthesis in prokaryotes have been identified and overexpressed. Of these, six are required for the thiazole biosynthesis (thiFSGH, thil, and dxs), one is involved in the pyrimidine biosynthesis (thiC), one is required for the linking of the thiazole and the pyrimidine (thiE), and f...

journal_title：Archives of microbiology

authors： Begley TP,Downs DM,Ealick SE,McLafferty FW,Van Loon AP,Taylor S,Campobasso N,Chiu HJ,Kinsland C,Reddick JJ,Xi J

更新日期：1999-04-01 00:00:00